The GC-Fluor® Technology
N.B. The following is designed to be a cursory and intuitive explanation of Ciencia's approach to SPR and SPCE. Small liberties have been taken for the sake of simplicity and to facilitate the appreciation of this technology as a biosensing platform. Interested parties are encouraged to seek out a more complete and precise presentation of SPR biosensing such as that found in Homola et al.1 Our Publications also provide considerable technical background supporting our approach to SPR, and constitute a considerable portion of the SPCE literature. We would also be happy to clarify any statements and answer any questions by email or by phone.
Surface Plasmon Coupled Emission
When the appropriate conditions are met, light directed
onto a metal surface will not reflect, but will instead excite a
surface plasmon and resonate. The excitation of fluorophores and the detection of their emission can be
greatly enhanced in the presence of this resonance. Our approach to achieving resonance is based on coupling with
a diffraction grating embossed on the sensor chip. For the more complete theory behind
SPCE, please see our initial paper on the demonstration of this phenomenon.2
The advantages of this grating-coupled SPCE approach to fluorescence detection can be thought of as arising
from two factors. First, the coupling of incident light into the plasmon permits a more efficient excitation
of the fluorophore. Second, and more importantly, the fluorescent emission in our system is not omnidirectional.
Instead, as is illustrated in the figure, the grating parameters "fold" the emitted light. These physical factors
combine with additional design elements to improve Ciencia's detection capabilities up to 100x over
standard fluorimeters. Our approach to SPCE permits femtomolar limits of detection at each of the 2,000 spots.
1 Homola J, Yee SS, Gauglitz G. (1999) Surface plasmon resonance sensors: Review. Sensors Actuators B: Chem 54(1–2):3-15.
2 Reilly Sr., M.T., Nessing, P.A., Guignon, E.F., Lynes, M.A., Fernandez, S.M. (2006) SPR surface enhanced fluorescence with a gold-coated corrugated sensor chip. Progress in Biomedical Optics and Imaging - Proceedings of SPIE Volume 6099, Article number 60990E DOI: 10.1117/12.646165